Journal: Animal Bioscience

Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits

doi: 10.5713/ab.24.0640

Figure Lengend Snippet: CXCL12 overexpression and knockdown in GCs. (A,B) Changes in the expression of CXCL12 after its overexpression and knockdown in GCs. (C,D) The mRNA expression level of PCNA , CCND1 , CDK2 , Bcl-2 and Bax was detected after overexpressing or knocking down CXCL12 in GCs. (E) The protein expression levels of CCND1, PCNA, Bcl-2 and Bax were detected after overexpressing and knocking down CXCL12 in GCs. H, high-litter size group; L, low-itter size group; GAPDH was selected as the internal reference gene (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001; ns, not significant). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, granulosa cells.

Article Snippet: Protein detection was achieved using the following antibodies: anti-CCND1 mouse monoclonal antibody (1:250, Proteintech), anti-PCNA rabbit polyclonal antibody (1:250, Proteintech), anti-Bcl2 rabbit polyclonal antibody (1:250, Proteintech), anti-Bax rabbit polyclonal antibody (1:250, Proteintech), anti-CITED1 rabbit polyclonal antibody (1:50, Proteintech), anti-WNT10B mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-CXCR4 mouse monoclonal polyclonal antibody (1:250, Proteintech), anti-phospho-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam, Cambridge, UK), anti-JAK2 rabbit monoclonal polyclonal antibody (1:250, Abcam), anti-phospho-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-STAT1 rabbit polyclonal antibody (1:250, Proteintech), anti-GAPDH mouse monoclonal antibody (1:2,500, Proteintech), 1:1,000 goat anti-rabbit secondary antibody IgG (Proteintech) and 1:1,000 goat anti-mouse secondary antibody IgG (Proteintech).

Techniques: Over Expression, Knockdown, Expressing

Journal: Nutrients

Article Title: Radix Rehmanniae Praeparata Extract Enhances Liver Regeneration Through AMPK-Driven Metabolic Reprogramming

doi: 10.3390/nu17223579

Figure Lengend Snippet: Different doses of RRP promote liver regeneration at 48 h post-PHx in mice. ( A ) Experimental design of PHx surgery, RRP administration (L, M, and H correspond to 2.5, 5, or 10 g/kg, respectively), and sample collection at 48 h post-PHx. ( B ) Liver coefficient. ( C ) Serum levels of ALT and AST. ( D ) H & E staining and immunohistochemistry of Ki67 and CCND1 in liver sections at 48 h post-PHx in the control and RRP-treated groups (2.5, 5, and 10 g/kg). Scale bar = 100 μm. ( E ) AOD quantification of KI67 and CCND1. ( F ) Relative mRNA expression of Ccnd1 and Ki67 in liver from each group was determined by qPCR and normalized using Hprt1 as an internal control. Statistical significance: ** p < 0.01, *** p < 0.001, **** p < 0.0001 as compared with the control group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 as compared with the PHx group ( n = 6 for mice).

Article Snippet: Paraffin sections underwent antigen retrieval; endogenous peroxidase blocking with 3% H 2 O 2 ; and overnight incubation with primary antibodies against Cyclin D1 (Ccnd1) (Proteintech, 1:100), Marker of proliferation Ki-67 (Ki67) (Proteintech, 1:600), and H3K27ac (CST, 1:100) in a wet chamber at 4 °C.

Techniques: Staining, Immunohistochemistry, Control, Expressing

Journal: Nutrients

Article Title: Radix Rehmanniae Praeparata Extract Enhances Liver Regeneration Through AMPK-Driven Metabolic Reprogramming

doi: 10.3390/nu17223579

Figure Lengend Snippet: RRP promotes early and sustained hepatocyte proliferation and alleviates liver injury after PHx. ( A ) Experimental timeline of 70% PHx and RRP treatment (5 g/kg). ( B ) Liver coefficient. ( C ) Serum levels of ALT and AST. ( D ) H & E, KI67, and CCND1 staining of liver sections at 24 h and 96 h post-PHx with or without RRP treatment. Scale bar = 100 μm. ( E ) AOD quantification of KI67 and CCND1. ( F ) Relative mRNA expression of Ccnd1 and Ki67 in liver, normalized to Hprt1. Statistical significance: ** p < 0.01, **** p < 0.0001 as compared with the control group; # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001 as compared with the PHx group without RRP treatment; $$$$ p < 0.0001 between indicated groups ( n = 6 for mice).

Article Snippet: Paraffin sections underwent antigen retrieval; endogenous peroxidase blocking with 3% H 2 O 2 ; and overnight incubation with primary antibodies against Cyclin D1 (Ccnd1) (Proteintech, 1:100), Marker of proliferation Ki-67 (Ki67) (Proteintech, 1:600), and H3K27ac (CST, 1:100) in a wet chamber at 4 °C.

Techniques: Staining, Expressing, Control

Journal: Nutrients

Article Title: Radix Rehmanniae Praeparata Extract Enhances Liver Regeneration Through AMPK-Driven Metabolic Reprogramming

doi: 10.3390/nu17223579

Figure Lengend Snippet: AMPK inhibition impairs RRP-induced metabolic remodeling and hepatocyte proliferation post-PHx. ( A ) Experimental timeline showing PHx, RRP administration (5 mg/kg), and Compound C (20 mg/kg) intervention. ( B ) Western blot analysis of pACC, ACC, pAMPK, and AMPK protein levels. ( C ) Hepatic acetyl-CoA levels. ( D ) Relative mRNA expression of Ccnd1 and Ki67 in liver from each group was determined by qPCR and normalized using Hprt1 as an internal control. ( E ) H&E and immunohistochemical staining for KI67, CCND1, and H3K27ac, with AOD quantitative analysis. ( F , G ) Serum levels of ALT and AST. Scale bar = 100 μm. Statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 as compared with the PHx group; # p < 0.05, ### p < 0.001, #### p < 0.0001 as compared with the PHx + RRP group ( n = 6 for mice).

Article Snippet: Paraffin sections underwent antigen retrieval; endogenous peroxidase blocking with 3% H 2 O 2 ; and overnight incubation with primary antibodies against Cyclin D1 (Ccnd1) (Proteintech, 1:100), Marker of proliferation Ki-67 (Ki67) (Proteintech, 1:600), and H3K27ac (CST, 1:100) in a wet chamber at 4 °C.

Techniques: Inhibition, Western Blot, Expressing, Control, Immunohistochemical staining, Staining